IN VITRO ANTI-OXIDANT AND IN VIVO ANTI -INFLAMMATORY ACTIVITY OF ETHANOLIC EXTRACT OF MIMUSOPS HEXANDRA
Vishavjeet*, B. K. Dubey, Vivek Singh Thakur, Deepak Basedia, Sunil Shah
Mimusops hexandra is a medicinal plant traditionally used for the treatment of various inflammatory and oxidative stress–related disorders. The present study aimed to evaluate the in vitro antioxidant and in vivo anti-inflammatory activity of the ethanolic extract of Mimusops hexandra. The ethanolic extract was prepared by solvent extraction and subjected to preliminary phytochemical screening, which revealed the presence of carbohydrates, flavonoids, proteins, and phenolic compounds. Quantitative analysis showed appreciable total phenolic content (0.843 mg/100 mg) and total flavonoid content (0.912 mg/100 mg) of the dried extract. The antioxidant activity was assessed using the DPPH free radical scavenging assay, where the extract exhibited concentration-dependent scavenging activity with an IC₅₀ value of 74.95 μg/ml, compared to ascorbic acid (IC₅₀ = 25.45 μg/ml). The anti-inflammatory activity was evaluated using the carrageenan-induced paw edema model in rats. The ethanolic extract, particularly at a dose of 200 mg/kg, showed significant inhibition of paw edema, especially during the later phase of inflammation, comparable to the standard drug indomethacin. The results suggest that the ethanolic extract of Mimusops hexandra possesses significant antioxidant and anti-inflammatory potential, which may be attributed to its phenolic and flavonoid constituents. The study supports the traditional use of Mimusops hexandra and indicates its potential as a natural therapeutic agent for managing oxidative stress and inflammatory conditions.
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